pegRNA pipeline
Author, private repo
Automated pegRNA design for prime-editing C-terminal protein tagging.
- PRIDICT 2.0
- Python
- Snakemake
- Imperial CX3 HPC
This pipeline designs pegRNAs for prime-editing C-terminal protein tagging. It supports the lab's work on tagging proteins in human cells, and it runs on the Imperial CX3 HPC cluster.
The design mode depends on the size of the insert. PE7 handles inserts up to 40 bp, TJ-PE handles up to 500 bp, and PA handles inserts over 1 kb. The pipeline picks the mode from the insert, so the person running it does not have to. It uses PRIDICT 2.0 to predict prime-editing efficiency.
The repository is private.